Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 28;120(49):e2306390120. doi: 10.1073/pnas.2306390120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

PMC Copyright notice

Fig. 2. — Characterization of nucleolin in HBV replication. siRNA against NCL were transfected into HepG2-NTCP cells; (A) mRNA and (B) protein levels of NCL were evaluated by qPCR (normalized to the siNT control) and western blot, respectively. (C) Five days after HBV infection, various HBV infection markers including cccDNA, HBV RNA (total), HBeAg, and HBV DNA in cell culture supernatant were determined and levels normalized to the siNT control. (D) The amount of HBV cccDNA was further determined by Southern blot. (E) HepG2-NTCP cells were transfected with siRNA against NCL or control and then transfected with or without plasmid overexpressing NCL. After HBV infection, HBeAg in cell culture supernatant were determined by ELISA. *P < 0.05 and ***P < 0.001.