FIG. 2.

Swarming abilities of HCB339 cells expressing the mutant Tar (A) or Tcp (B) protein as a sole chemoreceptor. Two microliters of fresh overnight culture was spotted onto tryptone semisolid agar supplemented with 50 μg of ampicillin per ml (A) or 25 μg of chloramphenicol per ml (B), and the plates were then incubated at 30°C for 12 h. (A) Tar[NWETF] (wild type), cells carrying pAK101; Tar[N], cells carrying pAK101-W550Op; None, cells carrying pBR322. (B) Tcp[NWESF], (wild type), cells carrying pAS101; Tcp[NWESLA], cells carrying pAS103; Tcp[NWES], cells carrying pAS104; Tcp[NWESC], cells carrying pAS105; Tcp[NWESA], cells carrying pOKU106; None, cells carrying pSU21.