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Dose-response relationship for the wild-type and mutant Tar proteins. HCB339 cells expressing the wild-type or mutant receptor were pretreated with 10% glycerol to reduce the SSF and were then exposed to various concentrations of aspartate. The SSF was measured within 1 min. (A) Tar[NWETF] (wild type), cells carrying pAK101; (B) Tar[N], cells carrying pAK101-W550Op.
