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. 1998 Apr;180(7):1862–1868. doi: 10.1128/jb.180.7.1862-1868.1998

FIG. 6.

FIG. 6

Expression levels (A and B) and methylation patterns (C and D) of the mutant Tar (A and C) and Tcp (B and D) proteins. (A and B) The wild-type or mutant receptors were expressed in the CheB− CheR− strain CP553 and detected by immunoblotting with anti-Tsr serum. Tar[NWETF] (wild type), cells carrying pAK101; Tar[N], cells carrying pAK101-W550Op; Tcp[NWESF] (wild type), cells carrying pAS101; Tcp[NWESLA], cells carrying pAS103; Tcp[NWES], cells carrying pAS104; Tcp[NWESC], cells carrying pAS105; None, cells carrying pSU21. (C and D) Cells expressing each receptor were preincubated with distilled deionized water (lanes D), 10 mM aspartate (lanes A), 10 mM citrate (lanes C), or 10% glycerol (lanes G). Chemoreceptors were detected as described for panels A and B. Tar[NWETF] (EEEE) CheB− CheR−, CP553 cells carrying pNI130; Tar[NWETF], HCB339 cells carrying pAK101; Tar[N], HCB339 cells carrying pAK101-W550Op; Tar[N] + CheR++, HCB339 cells carrying pAK101-W550Op and pRAR1 (a multicopy plasmid bearing cheR); Tar[N] + CheR+++, HCB339 cells carrying pAK101-W550Op and pKB23 (a CheR− overproducer plasmid), induced for CheR expression with 1 mM IPTG; Tcp[NWESF] CheB− CheR−, CP553 cells carrying pAS101; Tcp[NWESF], HCB339 cells carrying pAS101; Tcp[NWESLA], HCB339 cells carrying pAS103; Tcp[NWES], HCB339 cells carrying pAS104; Tcp[NWESC], HCB339 cells carrying pAS105; Tcp[NWESA], HCB339 cells carrying pOKU106.