TABLE 4.

D variants with amino acid substitutions at loops 3 and 4

D variant	D antigens/RBC*	Amino acid position and substitution
		Extracellular loop 3/transmembraneous helix 6								Transmembraneous helix 7/extracellular loop 4
		164	165	166	169	170	171	172	174	220	221	223	225	226	227	229
DDN†	ND	N	Y	H	M	M	H	I	V	W	P	F	S	A	L	R
DFL	7,499	D	C	H	M	M	H	I	V	W	P	F	S	A	L	R
DFW	10,019	D	Y	P	M	M	H	I	V	W	P	F	S	A	L	R
DFR-1	4,895	D	Y	H	L	R	H	F	V	W	P	F	S	A	L	R
DOL-1	7,158	D	Y	H	M	T	H	I	V	W	P	V	S	A	L	R
DMI	ND	D	Y	H	M	I	H	I	V	W	P	F	S	A	L	R
DHQ	ND	D	Y	H	M	M	Q	I	V	W	P	F	S	A	L	R
Weak D type 33	ND	D	Y	H	M	M	H	I	M	W	P	F	S	A	L	R
Weak D type 16	184	D	Y	H	M	M	H	I	V	R	P	F	S	A	L	R
Weak D type 62	ND	D	Y	H	M	M	H	I	V	W	T	F	S	A	L	R
Weak D type 27	409	D	Y	H	M	M	H	I	V	W	S	F	S	A	L	R
DFV	9,422	D	Y	H	M	M	H	I	VV	W	P	V	S	A	L	R
DTO	ND	D	Y	H	M	M	H	I	V	W	P	V	F	A	L	R
DSF‡	ND	D	Y	H	M	M	H	I	V	W	P	F	F	A	L	R
DCS-1	2,958	D	Y	H	M	M	H	I	V	W	P	V	S	P	L	R
DCS-2	835	D	Y	H	M	M	H	I	V	W	P	F	S	P	L	R
DBA	ND	D	Y	H	M	M	H	I	V	W	P	F	S	A	P	R
DHR	ND	D	Y	H	M	M	H	I	V	W	P	F	S	A	L	K
RhD consensus	NA	D	Y	H	M	M	H	I	V	W	P	F	S	A	L	R
RhCE consensus	NA	D	Y	H	L	R	H	F	V	W	P	V	S	P/A §	L	R

^*Samples were tested by flow cytometry with six monoclonal anti-D (BS221, BS227, BS228, BS229, BS231, and H41; Biotest). Weak D type 4.0 RBC run as a control showed 1689 D antigens/RBC. DFL (RIR-16) shown, 6310 D antigens/RBC measured for DFL of German donor. Hemizygous DOL-1 shown, 6154 D antigens/RBC measured for DOL-1/Ccde^s. The antigen densities of DCS-1 and DCS-2 were previously reported.²¹

^†The nucleotide sequence data were deposited in EMBL under Accession Number FM212439.

^‡Originally described as D674.²⁵

^§Amino acids P and A are typical for E and e antigens, respectively.

NA = not applicable; ND = not determined.