KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ZNF692 | Abcam | ab204595; RRID: AB_3068305 |
| RPA40 | Santa Cruz | sc-374443; RRID:AB_10991310 |
| FIBRILLARIN | Cell Signaling | #2639; RRID:AB_2278087 |
| FIBRILLARIN | Novus Biologicals | NBP2-26151; RRID: AB_3068306 |
| NPM1 | Santa Cruz | sc-47725; RRID:AB_628034 |
| FLAG | Sigma | F3165; RRID:AB_259529 |
| MYC | Abcam | ab32072; RRID:AB_731658 |
| Puromycin | Sigma | MABE343; RRID:AB_2566826 |
| α-Tubulin | Sigma | T6199; RRID:AB_477583 |
| β-Actin | Cell Signaling | #4970; RRID:AB_2223172 |
| NOP2 | Proteintech | 10448-1-AP; RRID:AB_2282772 |
| NHP2 | Proteintech | 15128-1-AP; RRID:AB_2267290 |
| EXOSC7 | Proteintech | 25292-1-AP; RRID:AB_2880011 |
| EXOSC8 | Proteintech | 11979-1-AP; RRID:AB_2101981 |
| KRR1 | Sigma | HPA043433; RRID:AB_10960988 |
| WBSCR22 (BUD23) | Thermo Fisher | PA5-62566; RRID:AB_2649539 |
| DIMT1 | Thermo Fisher | PA5-60170; RRID:AB_2640601 |
| RPS14 | Novus Biologicals | NBP2-22319; RRID: AB_3068307 |
| RPS16 | Novus Biologicals | NBP1-80025; RRID:AB_11002635 |
| RPS17 | Novus Biologicals | NBP2-93721; RRID: AB_3068308 |
| RPS18 | Novus Biologicals | NBP2-93632; RRID: AB_3068309 |
| RPL15 | ProteinTech | 16740-1-AP; AB_2301193 |
| Normal Rabbit IgG | Cell Signaling | #2729; RRID:AB_1031062 |
|
Chemicals, peptides, and recombinant proteins | ||
| Actinomycin D | Sigma | A9415 |
| GFP-nanotrap | Chromotek | Gta-10 |
| Harringtonine | Abcam | ab141941 |
| RNAse OUT | Thermo Fisher | 10777-019 |
| RNasin® Ribonuclease Inhibitor | Promega | N2115 |
| RNase A | Sigma | 10109169001 |
| DNase I | Sigma | 4716728001 |
| Click-IT™ AHA (L-Azidohomoalanine) | Thermo fisher | C10102 |
| Biotin Alkyne (PEG4 carboxamide-Propargyl Biotin) | Thermo fisher | B10185 |
| Click-iT™ Protein Reaction Buffer Kit | Thermo fisher | C10276 |
| 4-Thiouridine (4sU) | Sigma | T4509 |
| EZ-link HPDP Biotin | Thermo fisher | PIA35390 |
| Streptavidin Magnetic Beads | Pierce | 88816 |
| HRP-Conjugated Streptavidin | Thermo Scientific | N100 |
| SSC (20X), RNase-free | Invitrogen | AM9763 |
| UltraPure Ethidium Bromide | Invitrogen | 15585011 |
| Amersham Hybond-N+ membrane | GE Healthcare | RPN303B |
| Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate | Thermo Scientific | PI34076 |
|
Critical commercial assays | ||
| NorthernMax Kit | Invitrogen | AM1940 |
|
Deposited data | ||
| Proteomics | This study | Massive: MSV000089271 |
| RNA-seq | This study | GEO: GSE215838 |
|
Experimental models: Cell lines | ||
| Rat1 fibroblasts | Dr. John Sedivy (Brown University) | N/A |
| ARPE-19 | Sandra Schmid lab | N/A |
| HCT116 | ATCC | CCL-247 |
| DLD1 | ATCC | CCL-221 |
| RKO | ATCC | CRL-2577 |
| ARPE EV (pBabe-puro-HA) | This study | N/A |
| ARPE MYC (pBabe-puro-HA-MYC) | This study | N/A |
| HCT116 EV (pCMV-entry) | This study | N/A |
| HCT116 ZNF692 (pCMV-entry-ZNF692) | This study | N/A |
| HCT116 shControl (pLKO) | This study | N/A |
| HCT116 shZNF692-2 | This study | N/A |
| HCT116 shZNF692-5 | This study | N/A |
| DLD1 sgControl | This study | N/A |
| DLD1 sgZNF692-1 | This study | N/A |
| DLD1 sgZNF692-3 | This study | N/A |
|
Experimental models: Organisms/strains | ||
| NOD/SCID mice | Jackson lab | 001303 |
|
Oligonucleotides | ||
| universal siRNA controls #1 | Sigma mission | SIC001 |
| siRNA ZNF692-1 | Sigma mission | SASI_Hs01_00035045 |
| siRNA ZNF692-2 | Sigma mission | SASI_Hs01_00035047 |
| siRNA ZNF692-3 | Sigma mission | SASI_Hs02_00350781 |
| siRNA MYC-1 | Sigma mission | SASI_Hs01_00222676 |
| siRNA MYC-2 | Sigma mission | SASI_Hs01_00222677 |
| Primer and Northern probes | This study | Table S7 |
|
Recombinant DNA | ||
| pCMV-entry-ZNF692-Myc-DDK | Origene | RC200163 |
| pIRES-puro-GFP-ZNF692 | This study | N/A |
| pOCC29-TEV-GFP-ZNF692 FL | This study | JWV59 |
| pOCC29-TEV-GFP-ZNF692-ΔNt | This study | H079 |
| pOCC29-TEV-GFP-ZNF692-ΔZNF2-4 | This study | H080 |
| pOCC29-TEV-GFP-ZNF692-ΔNt-ΔZNF2-4 | This study | H081 |
| pLKO (control vector) | Sigma mission | SHC002 |
| ZNF692 shRNA-2 | Sigma mission | TRCN0000229714 |
| ZNF692 shRNA-5 | Sigma mission | TRCN0000219054 |
| EXOSC7 shRNA-1 | Sigma mission | TRCN0000051070 |
| EXOSC7 shRNA-2 | Sigma mission | TRCN0000051071 |
| EXOSC8 shRNA-1 | Sigma mission | TRCN0000303393 |
| EXOSC8 shRNA-2 | Sigma mission | TRCN0000051639 |
| KRR1 shRNA-1 | Sigma mission | TRCN0000072294 |
| KRR1 shRNA-2 | Sigma mission | TRCN0000072296 |
| NOP2 shRNA-1 | Sigma mission | TRCN0000157484 |
| NOP2 shRNA-2 | Sigma mission | TRCN0000154296 |
| Scrambled sgRNA CRISPR/Cas9 All-in-One Lentivector | Applied Biological Materials | K010 |
| ZNF692 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) | Applied Biological Materials | K2725906 |
| ZNF692 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) | Applied Biological Materials | K2725908 |
|
Software and algorithms | ||
| FIJI | ImageJ software | N/A |
| Prism 9 | Graphpad | N/A |
| Nucleolar localization sequence detector | Geoff Barton lab | N/A |
| GEPIA | http://gepia.cancer-pku.cn/about.html | Tang, Z. et al., 201743 |
| PrDOS: Protein DisOrder prediction System | https://prdos.hgc.jp/cgi-bin/top.cgi | Ishida T, Kinoshita K., 200744 |
| PyMOL | Schrodinger | N/A |
|
Other | ||
| RNA-seq TCGA | The Cancer Genome Atlas Program | N/A |
| RNA-seq Rat1 fibroblasts MYC OE | Maralice Conacci-Sorrell lab | Lafita-Navarro et al., 201819 |
| RNA-seq EμMyc mice | Bruno Amati lab | Sabo et al., 201422 |
| RNA-seq tet-off liver tumor mouse model | Bruno Amati lab | Kress et al., 201621 |
| ChemiDoc Imaging System | BIO-RAD | 17001401 |
| CFX96 Touch Real-Time PCR Detection System | BIO-RAD | 1855195 |
| BioLogic LP low-pressure chromatography system | BIO-RAD | 7318300 |
| Zeiss LSM780 Inverted confocal microscope | Zeiss | N/A |
| OMX SR Super-resolution Microscope | DeltaVision | N/A |
| Bioruptor Standard Sonication | Diagenode | UCD-200 |