Skip to main content
. 1998 Apr;180(7):1878–1886. doi: 10.1128/jb.180.7.1878-1886.1998

FIG. 5.

FIG. 5

Identification of transcription start points. (A) Primer extension analysis of 15 μg of total RNA from cells grown on ASWNH4 (NH4+) and ASWNO3 (NO3−). RNA was annealed to primer 9r and extended with reverse transcriptase. Sequencing was carried out on plasmid DNA containing the upstream region of the gene with the same primer and [α-32P]dATP. (B) Nucleotide sequence of the upstream region of ntcA from Synechococcus sp. strain WH 7803. The putative regulated transcription start point for cells grown on ASWNO3 is indicated by the arrow. The palindromic sequence and −10-promoter-like box are in uppercase and underlined. +1 corresponds to the first nucleotide of the GTG initiation codon. (C) RPA analysis of 30 μg of total RNA from cells grown on ASWNH4 (NH4+) and ASWNO3 (NO3−) with the upstream probe. The lengths of the transcript-probe duplexes are indicated. See Fig. 3 for the relevance of the yeast-containing lanes. The standards are the same as for Fig. 3.