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. 2023 Dec 9;14:8177. doi: 10.1038/s41467-023-44031-2

Fig. 3. KH-103-mediated nuclear translocation of GR.

Fig. 3

Immunofluorescent staining of GR in HEK293 (a) and A549 (b) cells treated with DEX or KH-103 for several durations. Scale bars are 100 μm. c Co-treatment of KH-103 with MG-132 in HEK293 cells at 30 min. Scale bars are 20 μm. d Relative mRNA expression of Sgk1 and Dusp1 in A549 cells treated with DEX, or KH-103 or DEX and KH-103 for 2 h (n = 3). For Sgk1, two-way ANOVA showed a significant main effect for KH-103 (F(1,8) = 34.77, p = 0.0004) and DEX(F(1,8) = 45.92, p = 0.0001). There was also significant interaction between KH-103 and DEX (F(1,8) = 27.47, p = 0.0008). Follow-up Dunnett’s multiple comparisons showed a significant difference between DMSO vs. DEX (p = <0.0001). For Dusp1, two-way ANOVA showed significant main effects for KH-103 (F(1,8) = 22.72, p = 0.0014) and DEX (F(1,8) = 91.40, p =<0.0001). There was also a significant interaction between KH-103 and DEX (F(1,8) = 30.06, p = 0.0006). Follow-up Dunnett’s multiple comparisons showed significant differences between DMSO vs. DEX (p = <0.0001) and DMSO vs. KH-103 + DEX (p = <0.0001). P-values ****<0.0001. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.