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. 2023 Dec 9;14:8177. doi: 10.1038/s41467-023-44031-2

Fig. 5. GR isoforms with intact LBD are degradable by KH-103.

Fig. 5

a Schematic depiction of plasmid used for expression of EGFP-GR fusion protein, immunoblot, and band quantification of HEK293 cells transiently expressing EGFP-GR and treated with KH-103. Unpaired two-tailed t test showed a significant difference between DMSO and KH-103 (t(4) = 7.08, p = 0.0021). b Schematic depiction of GR isoforms in fusion with HA and FKBP12F36V and their expression cassettes in dTAG plasmids. These proteins were targeted for degradation upon treatment with either KH-103 or dTAG13 and were detected by HA antibody. c Immunoblot of transiently expressed GR isoforms in A549 cells. *: unspecific band detected by HA antibody. d Band quantification and immunoblot of transcriptional GR isoforms transiently expressed in A549 cells treated with KH-103 or dTAG13. e Band quantification and immunoblot of translational GR isoforms transiently expressed in A549 cells treated with KH-103 or dTAG13. Results of ordinary one-way ANOVA and Holm–Sidak’s multiple comparison tests for transcriptional and translational isoforms are summarized in Supplementary Table 4. N = 3. P-values #<0.1, *<0.05, **<0.01, ***<0.001, ****<0.0001. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.