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. 2023 Dec 9;14:8177. doi: 10.1038/s41467-023-44031-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Heatmap of DEGs of the ligand-independent treatment groups plotted aside GR binding information ±2500 bp centered around their TSS, as well as ±2500 bp centered around their best predicted distal enhancer, after 2 h, 6 h, and 12 h DEX extracted from T. Reddy ChIP-seq data. (DEG analysis n ≥ 3/group; FDR < 0.05, |logFC| >log2(1.2)). b Heatmap of 13 DEGs triggered by KH-103 plotted aside GR binding information ±2500 bp centered around their TSS, as well as ±2500 bp centered around their best predicted distal enhancer, after 2 h, 6 h, and 12 h DEX treatment extracted from T. Reddy ChIP-seq datasets. (DEG analysis n ≥ 3/group; FDR < 0.05; |logFC| >log2(1.2)) c Volcano plot depicting GR as the only significantly differentially down-regulated proteins in A549 cells (n = 4/group) following 16 h 100 nM KH-103 treatment as assessed by label free proteomics. Dot size reflects number of peptides detected per protein. d Immunoblot and quantification of EGFP-IL6 transiently expressed in HEK293 cells treated with KH-103. N = 3. Ordinary one-way ANOVA showed no significant difference between DMSO and different KH-103 concentrations (F(3, 8) = 1.82, p = 0.2215). Follow-up Dunnett’s multiple comparisons tests also showed no significant differences. e Representative immunoblot and band quantification of MR in HEK293 cells treated with KH-103 at multiple concentrations. N = 4 independent experiments. Ordinary one-way ANOVA showed no significant difference between DMSO and different KH-103 concentrations (F(4,15) = 1.03, p = 0.4228). Follow-up Dunnett’s multiple comparisons tests also showed no significant differences. f Immunoblot and quantification of MPro fused with FKBP12^F36V transiently expressed in A549 cells treated with KH-103 and dTAG13. For both MPro-N and -C, n = 3 for DMSO, and n = 4 for KH-103 and dTAG13 treatments. Statistical details for the MPro (N) and (C) are summarized in Supplementary Table 4. P-values ^#<0.1, **<0.01. Data are presented as mean values ±SEM. Source data are provided as a Source Data file.