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. 2023 Dec 9;23:991. doi: 10.1186/s12903-023-03625-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The coculture model did not suppress the growth of DPCs. A Illustration of the DPCs and THP-1 coculture model and LPS treatment. B-C DPCs were cocultured with different ratios of THP-1 cells with 100 nM PMA and cultivated for 6 days. [B-1] THP-1: DPCs = 1:0 (pure THP-1 in THP-1 culture medium); [B-2] THP-1: DPCs = 0:1 (pure DPCs in THP-1 culture medium); [B-3] THP-1: DPCs = 1:1000 (in THP-1 culture medium); [B-4] THP-1: DPCs = 1:100 (in THP-1 culture medium); [B-5] THP-1: DPCs = 1:10 (in THP-1 culture medium); [B-6] THP-1: DPCs = 1:2 (in THP-1 culture medium); [B-7] THP-1: DPCs = 1:1 (in THP-1 culture medium); [B-8] THP-1: DPCs = 0:1 (pure DPCs in DPCs culture medium) (B) The cell morphology results. THP-1 cells and DPCs did not affect the morphology of each other. Magnification × 40. C The growth curve of cocultured DPCs and THP-1 cells. The growth trends of DPCs of [B-2] to [B-7] groups were similar in the coculture model. The data were expressed as the mean ± SD of triplicate wells form one experiment. ns, no significance; *, p < 0.05; **, p < 0.005; compared with the [B-2] THP-1: DPCs = 0:1 (pure DPCs in THP-1 culture medium) untreated control group. Two-way ANOVA