Fig. 4.

THP-1 enhanced more inflammatory response than LPS in the coculture model. DPCs were pretreated with 0 and 0.1 μg/mL LPS for 24 h, and cocultured with different ratios of THP-1 with 0.1 μg/mL LPS in THP-1 medium. A Cell morphology was photographed after 48 h. THP-1 cells were clustered in 0.1 μg/mL LPS-treated group. Triangle arrow – clustered THP-1 cells. Magnification × 100. B Total RNA was collected after 24 and 48 h. IL-6 and IL-8 mRNA expressions were determined by RT-qPCR. Cocultured with THP-1 increased IL-6 and IL-8 expressions at 24 and 48 h. 0.1 μg/mL LPS did not affect THP-1 induced IL-6 and IL-8 expressions at 24 h. But 0.1 μg/mL LPS increased THP-1: DPCs = 1:2-increased IL-6 and IL-8 expression at 48 h. The data were expressed as the mean ± SD of triplicates from three independent experiments. **, p < 0.005 compared with THP-1: DPCs = 0:; #, p < 0.05 compare with LPS 0 + THP-1: DPCs = 1:2 of each time point. Mann–Whitney test