Figure 6.

IAld suppressed NF-κB and JNK pathway through AhR in macrophage cells.

Notes: (A) RAW264.7 (B) THP-1 cells were treated with IAld (200 μM) for 1 h with or without 1 h prior treatment of CH-22 (10 μM), followed by LPS (100 ng/mL) stimulation for 30 min, the levels of phosphorylated NF-κB p65 (p-p65) and JNK (pJNK) were determined by immunoblot. The levels of phosphorylated NF-κB p65 (p-p65) were determined by immunoblot. Total protein was a loading control. Data are presented as the ratio of p-p65/total p65 and pJNK/total JNK. Representative images of immunofluorescent staining of p65 translocation from cytoplasmic to nucleus and quantitation of p65 translocated ratio in (C) RAW264.7 (D) THP-1. All data obtained from immunoblot or IF were quantified using Image J. The data are shown as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.

Abbreviation: ns, statistically non-significant.