FIG. 5.

Effects of mrsC505 on decay of the cat, kan, and secG mRNAs. RNA was isolated as described in the legend to Fig. 3. For analysis of the cat (A) and kan (C) transcripts, RNA was separated on 5% polyacrylamide gels. For secG (B), analysis on a 6% gel was used. The number above each lane indicates the time (minutes) after the temperature shift. (A) cat analysis, 7 μg of RNA/well; (B) secG analysis, 7 μg of RNA/well; (C) kan analysis, 7 μg of RNA/well for SK7961 (pnp-7 rnb-500 rne-1 [pSYK103 cat kan]) and 11 μg/well for SK8246 (mrsC505 pnp-7 rnb-500 rne-1 [pSYK103 cat kan]). The respective full-length transcripts for each mRNA are marked. Full-length transcripts are indicated by arrowheads on the left.