TABLE 1.
E. coli strains and plasmids used
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| C600 | leu thr | B. Bachmann |
| JC158 | serA HfrH | A. J. Clark |
| JC1557 | argG6 hisG1 leuB7 metB1 rpsL309 | A. J. Clark |
| MG1693 | thyA715 | CGSC |
| SK2145 | argG6 hisG1 leuB7 metB1 rpsL309 [pVK88B (Tcrqa-2+)] | pVK88B tranformat of JC1557 |
| SK2262 | argG6 hisG1 leuB7 metB1 rpsL309 zgj-203::Tn10 | S. R. Kushner |
| SK2732 | his-61 leuB7 metB1 rpsL309 mrsC505 mrsB1 [pVK88B (Tcrqa-2+)] | This study |
| SK4900 | leu thr zgj-203::Tn10 | C600 × P1SK2262 Tcr transductant |
| SK5662 | argG6 thyA715 zgj-203::Tn10 | MG1693 × P1SK4900 Tcr transductant |
| SK5665 | thyA715 rne-1 | 4 |
| SK5704 | thyA715 rne-1 rnb-500 pnp-7 | 4 |
| SK5726 | thyA715 rnb-500 pnp-7 | 4 |
| SK6828 | thyA715 mrsC505 | SK5662 × P1SK2732 ArgG+ transductant |
| SK7961 | SK5704/pSYK103 | This laboratory |
| SK7967 | MG1693/pSYK103 | This laboratory |
| SK8232 | thyA715 mrsC505 zgj-203::Tn10 | SK6828 × P1SK2262 Tcr transductant |
| SK8236a | thyA715 rne-1 rnb-500 pnp-7 mrsC505, zgj-203::Tn10 | SK5704 × P1SK8232 Tcr transductant |
| SK8238a | thyA715 rne-1 rnb-500 pnp-7 mrsC505 zgj-203::Tn10 | SK5704 × P1SK8232 Tcr transductant |
| SK8239 | thyA715 rne-1 rnb-500 pnp-7 mrsC505 zgj-203::Tn10 [pWK912 (KmrmrsC+)] | This study |
| SK8244 | thyA715 rne-1 mrsC505 zgj-203::Tn10 | SK5665 × P1SK8232 Tcr transductant |
| SK8246 | SK8238/pSYK103 | This study |
| SK8247 | SK8244/pSYK103 | This study |
| SK8248 | SK8236/pSYK103 | This study |
| SK8249 | SK5665/pSYK103 | This study |
| Plasmids | ||
| pSYK103 | Kmr Cmr, pSC101 derivative | This study |
| pVK88B | qa-2+ Tcr, pBR322 derivative | 1 |
| pWK912 | mrsC+ Kmr, pSC101 derivative | 49 |
a
Independently derived transductant.