TABLE 3.
Chemical half-lives of specific mRNAs
| Strain | Genotype | Half-life (min) + SDa
|
||
|---|---|---|---|---|
| trxAb | catc | kanc | ||
| SK7967 | Wild type | 3.5 ± 0.1 | 2.7 ± 0.2 | 3.5 ± 0.6 |
| SK8248 | mrsC505 | 6.4 ± 0.2 | 4.6 ± 0.5 | 6.6 ± 1.0 |
| SK8249 | rne-1 | 3.9 ± 0.1 | 4.5 ± 1.5 | 6.5 ± 0.1 |
| SK8247 | mrsC505 rne-1 | 8.2 ± 0.1 | 6.5 ± 1.5 | 9.7 ± 1.6 |
| SK7961 | pnp-7 rnb-500 rne-1 | 10.4 ± 0.4 | 32.7 ± 5.0 | 22.0 ± 1.5 |
| SK8246 | mrsC505 pnp-7 rnb-500 rne-1 | 49.0 ± 7.0 | >100 | 26.2 ± 0.9 |
a
Determined as described in Materials and Methods, using RNA-DNA dot blots. Each result is the average of at least two independent experiments.
b
Determined for a single chromosomal copy of gene.
c
On a plasmid present at six to eight copies/cell.