FIGURE 5.

Secretion kinetics of Bodipy sEV. Cells were pulsed with Bodipy C16 for 2 h and chased for different times. The number of purified sEV purified by differential ultracentrifugation was measured by (a) FC or by (b) Nanoparticle Tracking Analysis (NTA). Results are expressed as means  ±  SEM of at least 6 independent experiments. (c) Ratio between the total number of sEV counted by NTA and Bodipy sEV counted by FC. A constant ratio is showed up to 6 h, while there is an increase of total vesicles over the time. Results are expressed as means  ±  SEM of at least six independent experiments. (d) Representative Western blot analysis of tetraspanins expression in Bodipy sEV secreted at different chase time. The total sEV released at each time point has been analyzed.