Table 2.
The finest subsets alleviate high-resolution amplitude decay, along with a comparison to their theoretical number of particle limit
| Dataset | A | B | C | D | E | F | G | H |
|---|---|---|---|---|---|---|---|---|
| TRPA1 | 3.90 | 141.9 | 78.1 (63.8−) | 198.5 | 147.3 (51.2−) | 521 | 11,426 (21.9×) | 43,585 (83.7×) |
| hemagglutinin | 3.62 | 232.0 | 160.8 (71.2−) | 226.9 | 146.4 (80.5−) | 975 | 34,078 (35.6×) | 130,000 (133.3×) |
| LAT1 | 3.11 | 132.6 | 96.0 (36.6−) | 147.3 | 94.9 (52.4−) | 6697 | 65,687 (9.8×) | 250,712 (37.4×) |
| pfCRT | 3.37 | 85.1 | 49.5 (35.6−) | 235.8 | 66.8 (169.0−) | 4212 | 4429 (1.01×) | 16,905 (4.0×) |
| TSHR-Gs | 2.96 | 92.9 | 61.7 (31.2−) | 96.9 | 62.4 (34.5−) | 9205 | 13,465 (1.46×) | 41,054 (4.5×) |
| TRPM8 | 2.98 | 94.7 | 76.7 (18.0−) | 110.1 | 82.2 (27.9−) | 2200 | 13,789 (6.3×) | 42,040 (19.1×) |
| apoferritin | 1.81 | 70.5 | 58.0 (12.5−) | 81.6 | 49.2 (32.4−) | 74,530 | 80194 (1.08×) | 382,391 (5.1×) |
| streptavidin | 2.99 | 125.6 | 101.8 (23.8−) | 90.4 | 69.3 (21.1−) | 2152 | 7772 (3.6×) | 23,991 (11.1×) |
A Half-maps resolution of the CryoSieve-retained particles (Å); B B-factor reported by CryoSPARC auto-postprocessing obtained from all particles in the final stacks (Å2); C B-factor reported by CryoSPARC auto-postprocessing obtained from the CryoSieve-retained particles with temperature decrease (compared with all particles) in brackets (Å2); D Rosenthal’s B-factor obtained from all particles in the final stacks (Å2); E Rosenthal’s B-factor from the CryoSieve-retained particles with temperature decrease (compared with all particles) in brackets (Å2); F theoretical number of particles limit at B = 50 Å2; G number of the CryoSieve-retained particles with folds of theoretical limit in brackets.; H number of particles in final stacks with folds of theoretical limit in brackets. Three datasets (pfCRT, TSHR-Gs and apoferritin) were emphasized by bold font as the number of particle images in the finest subset approaches the theoretical limit.