Table 2.
Properties of prepared CaP nanoparticles. PDI: polydispersity index, measured with DLS.
| Sample (dispersion) | CaP/PEI/siRNA/SiO2 | CaP/PEI/pVEGF/SiO2 | CaP/PEI/pBMP-7/SiO2 | CaP/CMC |
|---|---|---|---|---|
| Solid core particle diameter by SEM/nm | 55 ± 7 | 83 ± 12 | 59 ± 9 | 71 ± 10 |
| V (one nanoparticle; only CaP)/m3 | 8.71·10−23 | 2.99·10−22 | 1.07·10−22 | 8.71·10−23 |
| m (one nanoparticle; only CaP)/kg | 2.73·10−19 | 9.40·10−19 | 3.37·10−19 | 2.73·10−19 |
| w(Ca2+) in the dispersion by AAS/kg m−3 | 0.098 | 0.073 | 0.076 | 25 wt% |
| w(Ca5(PO4)3OH) in the dispersion/kg m−3 | 0.246 | 0.183 | 0.191 | – |
| N(nanoparticles) in the dispersion/m−3 | 8.99·10+17 | 1.95·10+17 | 5.64·10+17 | – |
| Hydrodynamic particle diameter by DLS/nm | 206 | 93 | 126 | – |
| Polydispersity index (PDI) by DLS | 0.36 | 0.32 | 0.35 | – |
| Zeta potential by DLS/mV | +16 | +20 | +17 | – |
| N(pDNA)/m−3 | 2.59·10+22 | 2.02·10+22 | 2.15·10+22 | – |
| m(pDNA) per nanoparticle/kg | 6.46·10−19 | 2.93·10−18 | 9.92·10−19 | – |
| N(pDNA) molecules per particle | 2.88·10+4 | 1.04·10+5 | 3.81·10+4 | – |
| Endotoxin/EU·mL−1 | < 0.01 | 0.0133 | 0.0126 | <0.01 |