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. 2023 Nov 27;13:1294801. doi: 10.3389/fcimb.2023.1294801

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Copyright © 2023 Jeon, Han, Lee, Cho, Jang, Lee, Park, Kwon, Park, Kim, Lee, Kim and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Selection of a pair of anti-PirA and anti-PirB scFvs for developing an immunoassay. (A) CDR-H3 sequence and frequency of anti-PirA and anti-PirB scFv clones. Four anti-PirA scFvs (1B3, 1A8, 1C10, and 2C4) and two anti-PirB scFvs (3G7 and 3A5) were identified after sequencing analysis, and the frequency of each scFv antibody was determined. (B, C) Validation of binding activity of scFv clones. Each scFv with 6xHis tag was purified by Ni-NTA column and was titrated to assess the binding activity to (B) PirA, or (C) PirB by ELISA.