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. 2023 Nov 25;9(12):e22784. doi: 10.1016/j.heliyon.2023.e22784

Fig. 2.

Fig. 2

Detection of mtROS after P. g LPS and Kampo treatment. (A–E) Relative mtROS intensity was detected by mitoSOX. (A) NGF treatment (control). (B) P. g LPS treatment. (C) P. g LPS + Kamishoyosan treatment. (D) P. g LPS + Kamikihito treatment. (E) P. g LPS + Goreisan treatment. (F) Relative intensities of A–E. Relative mtROS were significantly increased after P. g LPS treatment. Pretreatment with Kamishoyosan or Kamikihito prevented the P. g LPS-induced changes in mtROS. Goreisan did not prevent the mtROS increase induced by P. g LPS. Fluorescent images were obtained from three separate dishes for each treatment. **: p < 0.01 vs. LPS by Scheffe’s F test.