Fig. 3.
Detection of mitochondrial membrane potential (Ψm) after P. g LPS and Kampo treatment. (A–E) Relative Ψm intensity was detected by JC-1. (A) NGF treatment (control). (B) P. g LPS treatment. (C) P. g LPS + Kamishoyosan treatment. (D) P. g LPS + Kamikihito treatment. (E) P. g LPS + Goreisan treatment. (F) Relative intensities of A-E. Relative Ψm was significantly decreased after P. g LPS treatment. Pretreatment with Kamishoyosan or Kamikihito attenuated P. g LPS-induced changes in Ψm. Goreisan did not prevent the Ψm decrease induced by P. g LPS treatment. Fluorescent images were obtained from three separate dishes for each treatment. **: p < 0.01 vs. LPS by Scheffe’s F test.