Antibodies |
|
PGAM5 |
Abcam |
Cat#126534 |
ATP5A |
Abcam |
Cat#14748 |
Rabbit Alexa Fluor Plus 488 |
Life Technologies |
Cat#A32731; RRID: AB_2633280
|
Mouse Alexa Fluor Plus 594 |
Life Technologies |
Cat#A32742; RRID: AB_2762825
|
|
Chemicals, peptides, and recombinant proteins |
|
McCoy’s 5A (modified) media |
Gibco |
Cat#16600082 |
Trypsin |
Gibco |
Cat#25200056 |
PBS |
Gibco |
Cat#14190144 |
16% Formaldehyde solution |
Thermo Scientific |
Cat#28908 |
Goat serum |
Gibco |
Cat#16210072 |
Fetal bovine serum |
Gibco |
Cat#26140079 |
CCCP |
Sigma |
Cat#C2759 |
DMSO |
Sigma |
Cat#41639 |
Penicillin-streptomycin |
Sigma |
Cat#P4333 |
Triton X-100 |
Bioshop |
Cat#TRX777 |
BSA |
Bioshop |
Cat#ALB001 |
Poly-D-lysine |
Sigma |
Cat#P6407 |
Fluoromount-G |
Invitrogen |
Cat#00495802 |
|
Experimental models: Cell lines |
|
U2OS |
Dr. Peter Kim’s lab, University of Toronto |
N/A |
|
Software and algorithms |
|
Las X |
Leica Microsystems |
https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
Fiji |
GitHub |
https://fiji.sc/ |
ImageJ |
NIH |
https://imagej.nih.gov/ij///index.html |
|
Other |
|
Microscope slides |
Fisherbrand |
Cat#22-037-246 |
1.5 mm glass coverslips |
Thermo Fisher Scientific |
Cat#12 518 213 |
Leica SP8 confocal microscope |
Leica |
N/A |