Complete IMDM medium for HMDMs:

Reagent	Final concentration	Amount
Heat-inactivated FBS	10%	50 mL
100 X pen/strep	1%	5 mL
Iscove’s Modified Dulbecco’s Medium (IMDM) (with L-glutamine and HEPES)	N/A	445 mL
Total	N/A	500 mL