REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Buffy coat blood or venous whole blood from healthy human volunteers	Human donors	N/A
Chemicals, peptides, and recombinant proteins
Recombinant human C5a	Sino Biological	10604-HNAE
Purified human C3a	Merck	US1204881
C5aR1 antagonists (PMX53, PMX205, JPE-1375)	Synthesized in-house (Li et al., 2020)	https://doi.org/10.1016/j.bcp.2020.114156
Bovine serum albumin	Sigma-Aldrich	A7906
Trypsin-EDTA (0.5%), no phenol red	Thermo Fisher Scientific	15400-054
UltraPure 0.5 M EDTA, pH 8.0	Thermo Fisher Scientific	15575020
Ham’s F12	Thermo Fisher Scientific	11765-062
Iscove’s modified Dulbecco’s medium (IMDM) (with L-glutamine and HEPES)	Thermo Fisher Scientific	12440053
Fetal bovine serum (FBS), heat-inactivated	Thermo Fisher Scientific	10082147
Penicillin-Streptomycin (10,000 U/mL)	Thermo Fisher Scientific	15140122
G418 (Geneticin)	InvivoGen	ant-gn-5
DPBS	Lonza	181828
10× DPBS	Lonza	181833
Water for cell culture applications	Lonza	181856
Lymphoprep	STEMCELL Technologies	07861
CD14 microbeads	Miltenyi Biotec	130-050-201
Critical commercial assays
AlphaLISA SureFire Ultra p-ERK1/2 Assay Kit	PerkinElmer	ALSU-PERK-A10K
Experimental models: Cell lines
CHO-hC5aR1	PerkinElmer	ES-731-C
CHO-hC3aR	PerkinElmer	ES-730-C
Software and algorithms
Prism 9	GraphPad	https://www.graphpad.com/features
Other
Corning 96 well clear bottom TC-treated microplates	Merck	CLS3596
ProxiPlate-384 Plus, white 384-shallow well microplate	PerkinElmer	6008280
SepMate PBMC isolation tubes	STEMCELL	85450
LS + positive selection column	Miltenyi Biotec	130-042-401
MidiMACS separator and multistand	Miltenyi Biotec	130-042-302
Sterilin 100 mm square Petri dishes	Thermo Fisher Scientific	109-17TS
Corning 96 well storage microplate	Merck	CLS3363
Falcon 40 μm cell strainer	Bio-Strategy	BDAA352340
Falcon cell scraper with 25 cm handle and 1.8 cm blade	Bio-Strategy	BDAA353086
Corning conical centrifuge tubes, sterile, 50 mL	Bio-Strategy	CORN430829
Alpha assay-capable multiplate reader (e.g., Tecan Spark 20M)	Tecan	https://lifesciences.tecan.com/multimode-plate-reader
Automatic cell counter (e.g., LUNA-II automated cell counter by Logos Biosystems)	Logos Biosystems	https://logosbio.com/luna-ll/
Suitable centrifuge (e.g., Beckman Coulter Allegra X-30 benchtop centrifuge)	Beckman Coulter	https://www.beckman.com/centrifuges/general-purpose/allegra-x-30#