Table 3.
Panel B (CD8+NKG2A/C+ T cells)
# | Specificity | Clone | Fluorochrome | ng/mL |
---|---|---|---|---|
1 | Live/Dead | - | Zombie UV | - |
2 | CD14 | 63D3 | APC-Fire 810 | 2.5 |
3 | CD19 | HIB19 | APC-Fire 810 | 0.625 |
4 | CD3 | SK7 | Spark Blue 550 | 2.5 |
5 | TCRyδ | 11F2 | BV480 | 2.5 |
6 | TCRVα2.4 | 6B11 | BV421 | 0.625 |
7 | TCRVα7.2 | 3C10 | APC-Cy7 | 1.25 |
8 | CD4 | OKT4 | BV510 | 1.25 |
9 | CD8 | RPA-T8 | BV570 | 1.25 |
10 | CD56 | QA17A16 | PE-Fire 640 | 1.25 |
11 | CD16 | 3G8 | Pacific Blue | 6.25 |
12 | CD45RA | HI100 | FITC | 2.5 |
13 | CCR5 | 2D7/CCR5 | BV750 | 2.5 |
14 | CCR7 | G043H7 | PerCP | 8 |
15 | CD26 | M-A261 | BUV395 | 2.5 |
16 | CD39 | A1 | PE-Fire 810 | 1.25 |
17 | CD73 | AD2 | BV711 | 1.25 |
18 | CD226 | DX11 | BUV737 | 2.5 |
19 | IL-21R | 17A12 | PE-Cy7 | 2.5 |
20 | IL-23R | 218213 | APC | 3 μLb |
21 | GITR | 621 | PE | 12 |
22 | NKG2A | S19004C | AF700 | 5 |
23 | NKG2C | 134591 | BUV615 | 2.5 |
24 | KIR2DL1/S1/S3/S5 (CD158) | HP-MA4 | BUV805 | 2.5 |
25 | KIR3DL1 (CD158e1) | DX9 | PerCP/Cy5.5 | 2.5 |
26 | CTLA4a | BNI3 | PE-Dazzle 594 | 3 |
27 | LAG-3 | 11C3C65 | BV785 | 3 |
28 | TIM-3 | F38-2E2 | BV650 | 3 |
29 | PD-1 | A17188B | PE-Fire 700 | 0.15 |
30 | Granzyme Ba | QA16A02 | PE-Cy5 | 0.3125 |
31 | Ki-67a | Ki-67 | AF647 | 6 |
Intracellular marker.
Recommended concentration by the manufacturer: 10 μL/106 cells.