REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Zombie UV™ Fixable Viability Kit	BioLegend	Cat#423107
APC/Fire™ 810 anti-human CD14	BioLegend	Cat#367156
APC/Fire™ 810 anti-human CD19	BioLegend	Cat#302271
Spark Blue™ 550 anti-human CD3	BioLegend	Cat#344851
BD OptiBuild™ BV480 Mouse Anti-Human γδ TCR	BD	Cat#746498
Brilliant Violet 421™ anti-human TCR Vα24-Jα18 (iNKT cell)	BioLegend	Cat#342915
APC/Cyanine7 anti-human TCR Vα7.2	BioLegend	Cat#351713
Brilliant Violet 510™ anti-human CD4	BioLegend	Cat#317443
Brilliant Violet 570™ anti-human CD8a	BioLegend	Cat#301037
PE/Fire™ 640 anti-human CD56 (NCAM) Recombinant	BioLegend	Cat#392431
Pacific Blue™ anti-human CD16	BioLegend	Cat#302024
FITC anti-human CD45RA	BioLegend	Cat#304105
BD Horizon™ BUV395 Mouse Anti-Human CD45RO	BD	Cat#564291
Brilliant Violet 650™ anti-human CD27	BioLegend	Cat#302828
PerCP/Cyanine5.5 anti-human CD28	BioLegend	Cat#302921
Brilliant Violet 711™ anti-human CD196 (CCR6)	BioLegend	Cat#353435
PerCP anti-human CD197 (CCR7)	BioLegend	Cat#353242
PE/Fire™ 810 anti-human CD183 (CXCR3)	BioLegend	Cat#353759
Brilliant Violet 750™ anti-human CD185 (CXCR5)	BioLegend	Cat#356941
BD OptiBuild™ BUV563 Mouse Anti-Human CXCR6 (CD186)	BD	Cat#748450
PE/Cyanine7 anti-human/mouse integrin β7	BioLegend	Cat#321241
PE/Fire™ 700 anti-human CD103 (Integrin αE)	BioLegend	Cat#350239
Alexa Fluor® 647 anti-human CD49d	BioLegend	Cat#304335
BD Pharmingen™ PE Mouse Anti-Human CD94	BD	Cat#555889
Alexa Fluor® 700 anti-human CD159a (NKG2A)	BioLegend	Cat#375120
BD OptiBuild™ BUV615 Mouse Anti-Human CD159C (NKG2C)	BD	Cat#751059
BD OptiBuild™ BUV737 Mouse Anti-Human CD314 (NKG2D)	BD	Cat#748426
PE/Dazzle™ 594 anti-human CD337 (NKp30)	BioLegend	Cat#325231
Brilliant Violet 785™ anti-human CD335 (NKp46)	BioLegend	Cat#331945
APC anti-human NKp80	BioLegend	Cat#346708
BD OptiBuild™ BV750 Mouse Anti-Human CD195 (CCR5)	BD	Cat#747224
BD OptiBuild™ BUV395 Mouse Anti-Human CD26	BD	Cat#744454
PE/Fire™ 810 anti-human CD39	BioLegend	Cat#328245
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase)	BioLegend	Cat#344025
BD OptiBuild™ BUV737 Mouse Anti-Human CD226	BD	Cat#748428
PE/Cyanine7 anti-human CD360 (IL-21R)	BioLegend	Cat#359513
Human IL-23 R APC-conjugated Antibody	Bio-Techne	Cat#FAB14001A-100
PE anti-human CD357 (GITR)	BioLegend	Cat#311603
BD OptiBuild™ BUV805 Mouse Anti-Human KIR2DL1/S1/S3/S5 (CD158)	BD	Cat#752514
PerCP/Cyanine5.5 anti-human CD158e1 (KIR3DL1, NKB1)	BioLegend	Cat#312717
PE/Dazzle™ 594 anti-human CD152 (CTLA-4)	BioLegend	Cat#369615
Brilliant Violet 785™ anti-human CD223 (LAG-3)	BioLegend	Cat#369321
Brilliant Violet 650™ anti-human CD366 (Tim-3)	BioLegend	Cat#345027
Alexa Fluor® 647 anti-human Ki-67	BioLegend	Cat#350510
PE/Fire™ 700 anti-human CD279 (PD-1)	BioLegend	Cat#621621
PE/Cyanine5 anti-human/mouse Granzyme B Recombinant	BioLegend	Cat#372225
Alexa Fluor® 700 anti-human CD27	BioLegend	Cat#302814
Alexa Fluor® 647 anti-mouse/rat/human FOXP3	BioLegend	Cat#320013
PE/Cyanine7 anti-human CD161	BioLegend	Cat#339917
PE anti-human CD25	BioLegend	Cat#302605
Brilliant Violet 650™ anti-human CD38	BioLegend	Cat#356620
PerCP/Cyanine5.5 anti-human HLA-DR	BioLegend	Cat#307629
APC anti-human HLA-E	BioLegend	Cat#342605
Brilliant Violet 421™ anti-human CD279 (PD-1)	BioLegend	Cat#329919
BD OptiBuild™ BV605 Mouse Anti-Human ULBP-2/5/6	BD	Cat#748131
BUV805 Mouse Anti-Human MICA	BD	Cat#749768
Experimental Models: Cell Lines
Frozen PBMCs from human donors isolated from fresh blood (information regarding sex, age, and other features of the donors is not available)	Établissement Français du Sang (France)	https://dondesang.efs.sante.fr/
Other
Human TruStain FcX™ (Fc Receptor Blocking Solution)	BioLegend	Cat#422301
Roswell Park Memorial Institute (RPMI) 1640 Medium	Life Technologies	Cat#21875034
Dulbecco's Phosphate Buffered Saline (1X)	Life Technologies	Cat#14190144
UltraComp eBeads™ Compensation Beads	Thermo Fisher Scientific	Cat#01-2222-42
BD Pharmingen™ Stain Buffer (BSA)	BD Pharmingen	Cat#554656
BD Horizon™ Brilliant Stain Buffer	BD Pharmingen	Cat#563794
BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit	BD Pharmingen	Cat#554714
Thermo Scientific™ Paraformaldehyde, 4% in PBS	Thermo Fisher Scientific	Cat#J61899
Sony ID7000TM spectral cell analyzer	Sony	https://www.sonybiotechnology.com/us/instruments/id7000-spectral-cell-analyzer/
Software and algorithms
Cytek Full Spectrum Viewer	Cytek® Biosciences 2019	https://spectrum.cytekbio.com/
FlowJo 10.7.1	© Becton Dickinson & Company (BD)	https://www.flowjo.com/
Spectral Flow Analysis (SFA) - Life Sciences Cloud Platform	Sony	https://www.sonybiotechnology.com/us/instruments/sfa-cloud-platform/