TABLE 3.
Sequence comparisons of isofunctional bacterial enzymes that catabolize chlorinated compounds
| Gene | Enzyme | Average % protein sequence identitya (no. of pairwise comparisons) | References |
|---|---|---|---|
| dhlA, dhaA | Haloalkane dehalogenase | 25.0 (1) | 23, 31 |
| dehC, hadL, dehH, dehH1, dehH2, dhlB, dehCI, dehCII | 2-Haloacid dehalogenase | 36.6 ± 3.9 (36) | 5, 25, 28, 29, 42, 43, 50, 59 |
| tfdA | 2,4-Dichlorophenoxyacetate monooxygenase | 43.2 ± 4.6 (21)b | 34, 37, 38, 56, 58 |
| dcmA | Dichloromethane dehalogenase | 56.0 (1) | 4, 32 |
| atzA | Atrazine chlorohydrolase | 98.6 ± 0.12 (15)c | This study |
| atzB | Hydroxyatrazine ethylaminohydrolase | 100 (10)c | This study |
| atzC | N-Isopropylammelide isopropylaminohydrolase | 99.0 ± 0.43 (10)c | This study |
a
All possible pairwise alignments of translated gene sequences were made. The average percent identity is the mean of the percent identity values for all pairwise alignments ± standard error of the mean.
b
Includes full protein sequences as well as partial protein sequences of ≥100 amino acids.
c
Sequence identity within a 0.5-kb PCR product for atzA and -B and within a 0.6-kb PCR product for atzC. Six sequences were analyzed for atzA, and five were analyzed for atzB and -C.