Figure 2.

Removal of DNA-linked protein during DPC repair via NER and HR. (A) Schematic of KCl-SDS-qPCR assay. KCl and SDS are used to selectively precipitate protein-crosslinked DNA. qPCR is then used to quantify the abundance of the DPC-containing fragment (red) relative to the control, non DPC-containing fragment (black). See text for details. (B) DPCs were transfected into cells proficient for NER (XPDC, HEK) or deficient for NER (XPD), in the presence of a heterologous or homologous donor plasmid. DPCs were recovered 1-h post-transfection and subjected to KCl-SDS-qPCR. Results depict % removal, measured by qPCR quantification of DPC-containing DNA, prior to and following transfection. *P= 0.01.