Figure 4.

Effect of proteasome inhibition on DPC removal mediated via NER and HR. NER-mediated removal was measured by transfecting DPC substrate into XPDC cells in the absence of a homologous donor, and HR-mediated removal was measured by transfecting DPC substrate into XPD cells in the presence of a homologous donor. Transfections were performed on cells that had been pre-treated for 1-h in the absence (–) or presence (+) of 10 μM MG132. Low molecular weight DNA was recovered one-h post-transfection, subjected to KCl-SDS-qPCR and % removal of DPC determined as described in the legend to Figure 2. *P= 0.05.