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Differential polyubiquitination patterns during NER and HR mediated repair. (A) DPCs were transfected into NER-proficient HEK293T cells and low molecular weight DNA recovered one-h post-transfection, then subjected to IP-qPCR using anti-K48 or K63 polyubiquitin -selective antibodies. (B) DPCs were transfected into NER-deficient XPD cells along with a non-homologous (–) or homologous (+) donor molecule and IP-qPCR performed as described above.
