Table 1.

Oligodeoxynucleotides (ODNs) used in this study. 8-oxoguanine (8-oxo0dG) containing sequences were annealed to M13mp18 and then extended using Taq polymerase to make double stranded, 8-oxoguanine containing M13, which was then crosslinked to OGG1 to make the DPC substrate

ODN	Sequence	Use
M13-8oxo	5′-AGGGTTTTCCCA(8-oxo-dG)TCACGACGTT-3′	Primer Extension
EcoRI-8oxo	5′-CCGGGTACCGAGCTC(8-oxo-dG)AATTCGTAATCTTGGTCATAGCTG-3′	Primer Extension
Fragment b L	5′-CACCCCAGGCTTTACACTT-3′	qPCR of M13 Plasmid
Fragment b R	5′-GTAAAACGACGGCCAGTG-3′	qPCR of M13 Plasmid
Fragment c L	5′-CTGGGTGCAAAATAGCAACT-3′	qPCR of M13 Plasmid
Fragment c R	5′-CCCAATAGCAAGCAAATCA-3′	qPCR of M13 Plasmid
DPC SSPE L	5′-GCTGCAAGGCGATTAAGT-3′	SSPEqPCR of M13 Plasmid
DPC SSPE R	5′-CGGCTCGTATGTTGTGTG-3′	SSPEqPCR of M13 Plasmid

See text for details. Depicted oligonucleotides were used for qPCR analyses. Fragment b and c respectively represent the amplicons found in the red and black M13 fragments depicted in the schematics shown in Figure 2A and Figure 3A. DPC SSPE represents the amplicon surrounding the DPC, depicted in the schematic shown in Supplemental Figure 1.