REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-AMPKα	Cell Signaling Technology	Cat#5831; RRID: AB_10622186
Rabbit monoclonal anti-phospho-AMPKα (Thr172)	Cell Signaling Technology	Cat#50081; RRID: AB_2799368
Rabbit monoclonal anti-GPX4	Abcam	Cat# ab125066; RRID: AB_10973901
Rabbit monoclonal anti-FTH	Abcam	Cat# ab75972; RRID: AB_1310223
Mouse monoclonal anti-FPN	Santa cruz	Cat# sc-518125
Mouse monoclonal anti-β-actin	Abcam	Cat# ab6276; RRID: AB_2223210
Rabbit monoclonal anti-GAPDH	Abcam	Cat# ab181602; RRID: AB_2630358
Secondary antibody goat anti-mouse IgG	Beverly	Cat#7074
Rabbit polyclonal anti-Ubiquitin	Cell Signaling Technology	Cat#3933S; RRID: AB_2180538
Chemicals, peptides, and recombinant proteins
minimum Eagle’s medium	Thermo Fisher Scientific	Cat#11095098
fetal bovine serum (FBS)	Sigma	Cat#12103C
Palmitic acid	Sigma	Cat# P0500
metformin	Sigma	Cat# PHR1084
erastin	Sigma	Cat# E7781
Ferric ammonium citrate	Sigma	Cat# F5879
interleukin-6	Cusabio	Cat# CSB-E04640r
ferritin	Cusabio	Cat# CSB-E08826r
protease inhibitors	Thermo Fisher Scientific	Cat# 36978
Bicinchoninic acid	Thermo Fisher Scientific	Cat# 23227
DAPI	Sigma	Cat# D9542
Lipofectamine 2000	Invitrogen	Cat#11668-019
Ferrostatin-1 (Fer-1)	Sigma	Cat# SML0583
chloroquine	Sigma	Cat# C6628
AICAR	Sigma	Cat# A1393
MG132	Sigma	Cat# M7449
Critical commercial assays
UltraSYBR Mixture RT-PCR reagent	CWBIO	Cat# CW2569M
Cell Counting Kit-8	Sigma	Cat# 96992
triglyceride	Nanjing Jiancheng Bioengineering Institute	Cat# A110-1-1
total cholesterol	Nanjing Jiancheng Bioengineering Institute	Cat# F002-1-1
malondialdehyde	Nanjing Jiancheng Bioengineering Institute	Cat# A003-1-2
glutathione	Nanjing Jiancheng Bioengineering Institute	Cat# A006-2-1
total superoxide dismutase	Nanjing Jiancheng Bioengineering Institute	Cat# A001-1-2
iron assay kit	Abcam	Cat# ab83366
Experimental models: Cell lines
WRL68	iCell Bioscience Inc	Cat# CL-48
Experimental models: Organisms/strains
Rats: Sprague-Dawley (SD)	Hunan Slac-Jinda Animal Company	N/A
Oligonucleotides
Primers for qRT-PCR, see Table S1	This paper	N/A
siRNA targeting sequence: FPN #1: GGATGGGTCTCCTACTACA	RiboBio	N/A
siRNA targeting sequence: FPN #2: GGACAAGAATGCTAGACTT	RiboBio	N/A
siRNA targeting sequence: FPN #3: GCACAGCTTTCCTGTTTGA	RiboBio	N/A
shRNA targeting sequence: AMPKα #1: GCGGCTCTTTCAGCAGATTCT	Honor Gene	N/A
shRNA targeting sequence: AMPKα #2: GCTGAAGTTTACCGAGCTATG	Honor Gene	N/A
shRNA targeting sequence: AMPKα #3: GCTGTGAAAGAAGTGTGTGAA	Honor Gene	N/A
Recombinant DNA
Plasmid: GFP-FPN	OriGene	Cat# RG205219
Software and algorithms
GraphPad prism 5	GraphPad	https://www.graphpad.com/
Other
Standard chow diet	Hunan Slac-Jinda Animal Company	N/A
High-fat diet	Hunan Slac-Jinda Animal Company	N/A