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. 2023 Nov 1;51(22):12185–12206. doi: 10.1093/nar/gkad934

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Expression levels of MSH2, MSH3 and MSH6 in different cellular contexts. Endogenous mRNA levels of MSH2 (red), MSH3 (blue) and MSH6 (green) were measured using RT-qPCR. (A) RNA was isolated from MSH3 or msh3Δ yeast cells at mid-log phase. (B) RNA was isolated at mid-log phase from msh3Δ strains carrying either an empty vector or either a low copy number (ARS CEN; LC) or a high copy number (2 micron; HC) plasmid, bearing MSH3 under the control of the endogenous MSH3 promoter. (C) RNA was isolated from strains co-overexpressing MSH2, under a constitutive promoter, and MSH3, under a galactose-inducible promoter. Culture aliquots were collected at indicated hours (h) after induction with galactose. All RNA levels were normalized to the reference gene PDA1. Data represents the mean of at least three independent experiments. Error bars represent SEM.