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. 2023 Nov 1;51(22):12185–12206. doi: 10.1093/nar/gkad934

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — MSH2 MSH3 overexpression induces a delay in cell cycle progression. MSH2 and MSH3 were overexpressed following galactose induction in a msh3Δ background. Aliquots were collected at 0 and 17 h after induction. (A) Histograms are shown of chromosomal content of asynchronous populations of MSH2 + empty vector (EV), MSH2 + MSH3 and MSH2 + MSH6 at several timepoints following addition of galactose. 1C indicates 1× DNA content; 2C indicates 2x DNA content. (B) Quantification of relative proportion of cells in different phases of the cell cycle for MSH2 + EV, MSH2 + MSH3 or MSH2 + MSH6. The percentage of cells in G1 (1C), G2/M (2C) or S (between 1C and 2C) phases was determined using FlowJo software (see Supplementary Figure S2 for details). Plotted values correspond to data collected from at least three independent experiments from at least two independent isolates. Error bars represent SEM. Pairwise t-tests indicated significant differences in the number of cells in G1 and S phase between MSH2 EV and MSH2 MSH3 O/E (G1, P< 0.0001; S, P = 0.0026) and between MSH2 MSH3 O/E and MSH2 MSH6 O/E (G1, P = 0.0023; S, P = 0.0011).