Skip to main content
. 2023 Nov 13;42(24):e114889. doi: 10.15252/embj.2023114889

Figure EV1. Sequence alignments of yeast Lac1 and Lag1, and human CerS family members.

Figure EV1

Secondary structural elements of yeast Lac1 are labeled above the sequence alignment. CH: cytosolic helix; TM: transmembrane helix; LH: luminal helix. The NTD is colored wheat. The TLC domain is colored marine with the Lag1p motif shown in yellow. The four red triangles indicate the predicted catalytic histidine and aspartate residues in the Lag1p motif. The Hox domains and acyl‐CoA selective regions in human CerS are highlighted with wheat and dark pink boxes, respectively. The DxRSDxE dimerization motif is highlighted with a green box. The UniProt IDs for the aligned sequences are as follows: yLac1: P28496; yLag1: P38703; hCerS1: P27544; hCerS2: Q96G23; hCerS3: Q8IU89; hCerS4: Q9HA82; hCerS5: Q8N5B7; and hCerS6: Q6ZMG9. “y” for Saccharomyces cerevisiae (yeast) and “h” for Homo sapiens (human).