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. 2023 Nov 13;42(24):e114889. doi: 10.15252/embj.2023114889

Figure EV2. Biochemical characterization of the Lac1‐Lip1 and Lag1‐Lip1 complexes.

Figure EV2

  1. Size exclusion chromatography (SEC) profiles and Coomassie blue‐stained SDS‐PAGE gel of the purified Lac1‐Lip1 and Lag1‐Lip1 complexes.
  2. Enzymatic activity of the WT Lag1‐Lip1 complex and the catalytic mutant Lag1H255A/H256A‐Lip1. Each data point is the average ± SEM of three independent experiments.
  3. CerS activity versus DHS concentration for the Lac1 alone protein and the Lac1‐Lip1 complex. The activity curve of the Lac1‐Lip1 complex follows a Michaelis–Menten equation with a K m of 39.44 ± 6.29 μM for DHS and a V max of 593.3 ± 34.5 nmol/min/mg. Each data point is the average ± SEM of three independent experiments.
  4. SEC profile and Coomassie blue‐stained SDS‐PAGE gel of the Lac1 alone protein and the Lac1‐Lip1 complex.