Figure EV5. Human CerS structural models predicted by AlphaFold.

1. The AlphaFold‐predicted human CerS structures (hCerS1AF‐hCerS6AF). The N‐terminal regions and Hox domains of hCerSs are colored light gray. The TLC domains are colored green with the acyl‐CoA selective regions highlighted in dark pink. The predicted catalytic histidine and aspartate residues in the Lag1p motifs are shown in sticks.
2. Structural superposition of the hCerS5AF (colored green) and the Lac1‐Lip1 heterodimer (colored wheat and light cyan). The N‐terminal region of hCerS5AF severely clashes with the TM of Lip1.