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. 2023 Nov 20;42(24):e113240. doi: 10.15252/embj.2022113240

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© 2023 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure EV4 — A–C
Immunoblot analysis of lysates from WT and zuo1Δ cells containing TIF1‐5xFLAG (A), TIF2‐5xFLAG (B), or CDC33‐5xFLAG (C) at the endogenous locus treated with 200 nM rapamycin for 2 h or left untreated. Ponceau and Pgk1 staining served as the loading control.

D
Fivefold serial dilutions of the indicated strains grown on YEPD plates with or without 20 ng/ml rapamycin for 4 days at 30°C.

Source data are available online for this figure.