Fig. 2.

Bone marrow characterization of mice MSCs in vitro. Phenotype of BM-MSCs were assessed via flow cytometry analysis, which revealed that the cells expressed CD73, CD90, and CD105 and did not express the hematopoietic markers CD34, CD45, and CD11b A. Following 21 days of incubation in adipogenic medium, adipocytes were identified by accumulation of large fat droplets and staining for lipid with Oil Red O under light microscopy in cells. A. Also, the osteoblast differentiation of mice BM-MSCs was monitored under a light microscope C. At day 21, the calcified nodules of mice BM-MSCs were visualized by alizarin red S staining