Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 10;14:358. doi: 10.1186/s13287-023-03578-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Flow cytometric analysis to evaluate the effect of engrafted MSCs on the frequency of splenocytes. A representative gating scheme and representative dot plots are also presented. Th1, Th2, Th17, and Treg percentages were significantly higher in the P–C group than in the N–C group. The population of Th1 and Th2 in all MSCs treated groups was significantly lower than in the P–C group (A and B). Transplantation of naïve MSCs and pre-exposure MSCs to L. delbrueckii significantly decreased the percentage of Treg and Th17 cells compared with the P–C group. However, there was no significant difference between the percentage of Th17 and Treg cells of mice treated with pre-exposure MSCs to L. rhamnosus with those of the P–C group. Furthermore, the percentage of Th17 cells in the DR-MSCs group was significantly reduced. However, no significant differences were observed regarding the percentage of Treg cells between the DR-MSCs and P–C groups. These findings support the hypothesis that bacteria can interact with MSCs and educate MSCs with potent immunosuppressive and immunomodulatory properties. Interestingly, a mix of bacteria did not have a similar effect as R-MSCs or D-MSCs. Tukey’s multiple comparisons were used to determine the relationship between the variable’s means. N–C: Negative Control (Healthy mice treated with PBS); P–C: Positive Control (Pristane-immunized mice treated with PBS); N-MSCs (Naïve MSCs without any interventions); R-MSCs: MSCs exposed to lactobacillus rhamnosus; D-MSCs: MSCs exposed to lactobacillus delbrueckii; DR-MSCs: MSCs exposed to a mixture of lactobacillus rhamnosus and delbrueckii. Data were presented as Mean ± Standard Error of the Mean (SEM). P values of ≤ 0.05 were considered significant. (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001)