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. Author manuscript; available in PMC: 2023 Dec 11.
Published in final edited form as: Cell Rep. 2023 Aug 4;42(8):112928. doi: 10.1016/j.celrep.2023.112928

Figure 2. Expression of the miR-23–27-24 clusters promotes LAM accumulation in obese eWAT.

Figure 2.

(A) Representative flow panels identifying ATMs from obese fl/fl and MyelΔ mice.

(B) Myeloid cell numbers were compared between obese fl/fl and MyelΔ mice (two-way ANOVA with Šídák’s multiple comparison test). ATE, adipose tissue eosinophil; ATM, adipose tissue macrophage; ATN, adipose tissue neutrophil.

(C and D) mRNA levels in whole eWAT (C) or serum levels (D) of IL-6 and TNF-α were compared between obese fl/fl and MyelΔ mice (two-tailed t test).

(E–G) Quantification of M1-like and M2-like markers on ATMs via flow cytometry; two-tailed t test for comparison of (E) and (F), two-way ANOVA with Šídák’s multiple comparison test for (G).

(H and I) Expression of CD9 on ATMs (H) and number of CD9+ ATMs (I) by flow cytometry (two-tailed t test).

(J) Trem2 mRNA levels from whole fl/fl and MyelΔ eWAT compared via RT-qPCR (one-sample t test).

(K) Representative image of eWAT sections stained with anti-F4/80 to visualize crown-like structures (orange star) with quantitation (one-tailed t test). Each dot represents one male mouse after HFD feeding. ±SEM. *p ≤ 0.05, ***p < 0.001, ****p < 0.0001. See also Figures S2 and 3.