FIGURE 2.

(A) Particle concentrations of bdEVs from brain regions were measured using NFCM. The particle concentration for each region was normalized by tissue mass (per 100 mg). (B, C) bdEVs were visualized by negative staining transmission electron microscopy (TEM) at 60, 000× and 100,000× magnification, separately; scale bar = 500 nm in (B), and 100 nm in (C). TEM is representative of 10 images taken of each region. (D) Size (diameter) distributions of bdEVs from brain regions as measured by NFCM and calculated as particles in each 5 nm size bin versus total detected particles in each sample (percentage). (E) Size distributions of bdEVs from brain regions as measured in TEM images and calculated as particles in each 50 nm size bin versus total detected particles in each sample (percentage).