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EV surface protein phenotyping. CD63, CD81, and CD9 were detected on the intact bdEV surface by single‐particle interferometric reflectance imaging sensor (SP‐IRIS) (A) and multiplexed ELISA (B) and normalized per 100 mg tissue input. bdEVs were captured by antibodies to EV membrane proteins and detected by a signal from a cocktail of anti‐tetraspanin antibodies (CD63, CD81, and CD9).
