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. 2023 Dec 5;53(1):10. doi: 10.3892/ijmm.2023.5334

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Copyright: © Jang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of MT RANKL on NFATc1 nuclear translocation. (A) NFATc1 nuclear translocation in LGR4 siRNA-transfected BMDMs was analyzed in the cytoplasmic and nuclear fractions. Histone-H1 and β-actin were used as loading controls for the nuclear and cytoplasmic fractions, respectively. (B) Densitometric analysis of NFATc1 expression in the cytoplasmic and nuclear fractions of LGR4 siRNA-transfected BMDMs is presented as the mean ± standard deviation of three separate experiments. (C) NFATc1 nuclear translocation in BMDMs pretreated with LiCl for 8 h before RANKL treatment. (D) Densitometric analysis of NFATc1 expression in BMDMs pretreated with LiCl. The results are representative of three separate experiments that had comparable data. Data are presented as the mean ± standard deviation. ^*P<0.01 vs. control group; ^#P<0.01 vs. WT RANKL. BMDM, bone marrow-derived macrophage; CTL, control; LGR4, leucine-rich repeat-containing G-protein-coupled receptor 4; LiCl, lithium chloride; MT, mutant; NFATc1, nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 1; N.S., not significant; RANKL, receptor-activated nuclear factor-κB ligand; si, small interfering; WT, wild-type.