Figure 3.

Transcriptome analysis of different brain laminae. (A) Schematic view of brain tissue samples at E80, E110, E133 and P60 (scale bar, 1 cm). (B) Counts of the up-regulated and down-regulated DEGs of different laminae in the TG monkeys. (C) Volcano plot at E80 showing DEGs of CP, OSVZ, SVZ and VZ. Left panel, the laminar structure of the monkey cortex at E80 (|log2FC| > 0.7, padj < 0.05). (D–G) GO enrichment terms of (D) CP, (E) OSVZ, (F) SVZ and (G) VZ at E80. (H) The PPI network of the up-regulated genes at E80 constructed using STRING. The outer circle depicts distinct colors indicating various GO enrichment terms. The color and thickness of the arrows reflect the interaction score. (I) Immunofluorescence staining for Nkx2.2 and Olig2, combined with DAPI, was performed using the WM samples of DLPFC at P60 (scale bar, 250 μm). (J) Quantification of Nkx2.2⁺ and Olig2⁺ cells normalized by DAPI⁺ at P60 in a 400 μm × 400 μm square region of the DLPFC WM. Two monkeys were analyzed for each group (TG or WT), covering nine regions per monkey (unpaired Student's t test, ***P < 0.001).