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SDS-PAGE of 4NBZDH on a 12% polyacrylamide gel. Lane 1, crude cell extract of TW3 prior to loading onto the dye affinity column; lanes 2 to 5, successive fractions containing 4NBZDH eluted from the column with 1 mM NAD+. Marker sizes are indicated in kilodaltons. The molecular mass of the major band is ∼53 kDa.
