TABLE 1.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Genotype or phenotypea | Reference or source |
|---|---|---|
| Pseudomonas sp. strain TW3 | 4NT+ 4NBA+ 4NBZate+ | 27 |
| Pseudomonas sp. strain PaW130 | Rifr | 15 |
| E. coli XL1-Blue MRF′ | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac(F′ proAB lacIqZ M15 Tn10 [Tetr]) | Stratagene |
| E. coli TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7679 galU galK rpsL(Strr) endA1 nupG | Invitrogen |
| E. coli BL21(DE3)/pLysS | F−ampT hsdSB (rB− mB−) dcm gal λ(DE3) pLysS(Cmr) | Promega |
| pCK04 | Cmr; derivative of pSC101 containing pWW0 xylUWCMABN on a ∼15-kbp NotI fragment | 17 (GenBank accession no. for operon, D63341) |
| pTW3.1 | 7.5-kbp HindIII clone containing ntnWCMAB* in pUC18 | This study |
| pTW3.1E | 4.5-kbp EcoRI/HindIII subclone of pTW3.1 in pUC18 | This study |
| pTW3.5 | 4.3-kbp XhoI clone containing ntnWCM in pUC18 (cloned into SalI site) | This study |
| pTW3.6 | 6-kbp EcoRI clone containing ntnCMAB* in pUC18 | This study |
| pETntnC | NdeI/EcoRI fragment containing ntnC in pET5a | This study |
a
4NT, 4-nitrotoluene; 4NBA, 4-nitrobenzyl alcohol; 4NBZate, 4-nitrobenzoate.