Skip to main content
. 2023 Dec 11;12:RP91263. doi: 10.7554/eLife.91263

Figure 2. Sonic hedgehog (Shh) is expressed in mossy cells and upregulated upon seizure induction.

(A) Representative immunofluorescence images of mossy cells labeled with GluR2/3 (green) and ShhCre/+;RosaAI14 (tdTomato; red) 1 week after seizure induction. (B) Quantification of tdTomato+ cells in each area of the dentate gyrus (DG). GCL: granule cell layer, ML: molecular layer (naive: n = 3, kainic acid [KA]: n = 3 mice). (C) Representative immunofluorescence images of interneurons labeled with parvalbumin (cyan) and ShhCre/+;RosaAI14 (tdTomato; red) in the hilus. (D) Cell-type population of tdTomato+ cells in the hilus of ShhCre/+;RosaAI14 mice 1 week after seizure induction (naive: n = 3, KA: n = 3 mice). (E) In situ RNAscope detection of Shh mRNA (green) in the DG from anterior to posterior 24 hr after seizure induction. (F) Representative RNAscope-immunofluorescence images for Shh expression in mossy cells labeled with GluR2/3 (red). (G) Quantification of Shh mRNA puncta in GluR2/3+ mossy cells. Values represent mean ± standard error of the mean (SEM); ****p < 0.0001. Unpaired t-test (two-tailed). A total of 608 and 584 GluR2/3+ cells were quantified from three mice in naive and KA treated groups, respectively.

Figure 2—source data 1. Raw data for counts.

Figure 2.

Figure 2—figure supplement 1. Shh is expressed in dorsal mossy cells.

Figure 2—figure supplement 1.

(A) Schematic illustration of coronal sections of hippocampus (reference images of the coronal mouse brain from Allen Brain Atlas). (B) Representative immunofluorescence images of dentate gyrus (DG) from ShhCre/+;RosaAI14 mice 1 week after seizure induction. Higher magnification of inset panels from dorsal and ventral regions of posterior DG. GluR2/3+ (green) mossy cells in dorsal but not ventral DG were labeled with ShhCre/+;RosaAI14 (red).