Figure 6. Deletion of Shh in mossy cells increases age-related NSC decline.
(A) Representative immunofluorescence images of Sox2+ (green) GFAP+ (red) radial NSCs in 9- to 11-month-old control and Shh-cKO mice. High magnification of inset images are shown in the right panels. (B) Quantification of Sox2+ GFAP+ radial NSCs. Values represent mean ± standard error of the mean (SEM); *p < 0.05. Unpaired t-test (two-tailed, control: n = 4, Shh-cKO: n = 3 mice). (C) Experimental scheme of long-term tracing of proliferating NSCs in aged mice. Nine- to eleven-month-old control and Shh-cKO mice were administrated with 5-bromo-2′-deoxyuridine (BrdU) via drinking water for 5 days and were analyzed on the last day of BrdU administration (day 0), and at 10 days and 4 weeks after BrdU administration. Schematic of NSC fate after proliferation. (D) Representative immunofluorescence images of proliferating Sox2+ (green) NSCs labeled with BrdU (cyan) at day 0 and radial Sox2+ (green) GFAP+ (red) NSCs retaining BrdU (cyan) at 10 days and 4 weeks after BrdU administration. (E) Quantification of the fraction of NSCs retaining BrdU until 10 days and 4 weeks after BrdU administration in aged control and Shh-cKO mice. Values represent mean ± SEM; *p < 0.05, **p < 0.01. Unpaired t-test (two-tailed, 10 days: control: n = 3, Shh-cKO: n = 4, 4 weeks: control: n = 5, Shh-cKO: n = 5 mice mice).
Figure 6—figure supplement 1. Expressing Designer Receptors Exclusively Activated by Designer Drugs (DREADD) activator does not affect neurogenesis and number of NSCs of Crlr-Cre Shh mice.
